| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 181 | Treponema pallidum | GCATTCACAATGGCGTGGT | GGACCAAACACTCCTCGCA | 59.41 | 59.93 | 241 | 61 |
100.00%
|
100.00%
|
| Primer 182 | Treponema pallidum | AGACACTTGGCATGCGCT | ACGTCTTTGCTCCAGGCT | 59.97 | 58.85 | 191 | 61 |
100.00%
|
100.00%
|
| Primer 183 | Treponema pallidum | ACGCTCGTGCCATTGCT | TGTAACAACCGCTCGGCA | 60.01 | 59.58 | 193 | 61 |
100.00%
|
100.00%
|
| Primer 184 | Treponema pallidum | TGCTCAGATGGGTTGCCA | ATACCCCAACGCAACGCT | 58.83 | 59.65 | 233 | 61 |
100.00%
|
100.00%
|
| Primer 185 | Treponema pallidum | CCGTTGCGTTACACAGCAC | TCGGCAAGTTCAGGCACA | 60.08 | 59.49 | 259 | 59 |
100.00%
|
100.00%
|
| Primer 186 | Treponema pallidum | AGGCTCATCGTGGAAAGGG | TGCGCATCCAAACGTCTCT | 59.40 | 60.01 | 178 | 59 |
100.00%
|
100.00%
|
| Primer 187 | Treponema pallidum | TGCTGCTCGCATTAACGC | TGCTGCGCACATGTAGCA | 59.22 | 60.36 | 294 | 58 |
100.00%
|
100.00%
|
| Primer 188 | Treponema pallidum | ATGCGCAGGGCTGTTTGA | ACGACGCAGATGCTCCTT | 60.28 | 59.03 | 287 | 58 |
100.00%
|
100.00%
|
| Primer 189 | Treponema pallidum | GCATTCACAATGGCGTGGT | TGGACCAAACACTCCTCGC | 59.41 | 59.93 | 242 | 58 |
100.00%
|
100.00%
|
| Primer 190 | Treponema pallidum | ACTCCGACGAAGACCCCTT | ATATCGGCTGCCTGTTCGG | 60.23 | 59.93 | 277 | 58 |
100.00%
|
100.00%
|